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肾移植术后测定外周血CD3/HLA

  Obiective To investigate the changes in the activated T-cell populations CD3/HLA-DR and CD3/CD(16+56) in peripheral blood in the patients with kidney transplantation.Methods The lymphocytes from the peripheral blood in the patients in different periods after kidney transplantation were immunologically labeled by dual color fluorescent monoclonal antibodies CD3/HLA-DR and CD3/CD(16+56)CD3/HLA-DR and CD3/CD(16+56)were determined by using flow cytometry(FCM).Results The levels of CD3+/CD(16+56)+,CD3-/CD(16+56)+ and CD3-/HLA-DR+ in the patients before renal transplantation were higher than in the normal subjects,while the CD3+/CD(16+56)- and CD3+/HLA-DR- were lower.Three days after transplantation,the T cells and T cell subsets were decreased in the getting-well patients,and the CD3+/CD(16+56)+ were the most Obvious among them.The levels of CD3-/CD(16+56)+,CD3+/CD(16+56)+and CD3-/HLA-DR+ were significantly increased in the group with rejection. The four parameters mentioned above in patients with acute nephrotubal necrosis were all abnormally increased as two times as those of the group with rejection.Conclusion The dynamic determination of CD3/HLA-DR and CD3/CD(16+56)is helpful to the early diagnosis and differential diagnosis of acute kidney rejecuon from acute nephrotubal necrosis.and is of some important for the treatment of rejections and the evaluation of the curative effects of antirejection.

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